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NBRP Fundamental Technologies Upgrading Program Project's Progress

Drosophila

Focus Development of cryopreservation method of Drosophila strains
Principal Investigator Ryu Ueda
Genetic Resource Center,National Institute of Genetics
Project Period FY2012–2013
Overview   The National BioResource Project “Drosophila” (NBRP Drosophila) is conducted by consortium composed of five research institutes and universities that function as one of the largest Drosophila stock centers in the world. Despite the various efforts made historically, efficient techniques for cryopreservation of Drosophila stocks have not been established. By contrast, along with recent years’ development of genome information and genetic engineering technology, large-scale, systematic mutant production projects are ongoing. The reality is that only a limited number of stock centers exist worldwide and are struggling for Drosophila stock preservation.
  This program aims to develop a cryopreservation technique for Drosophila stocks. While the cryopreservation of ova (embryos) or sperm has been attempted so far, the preservation of larval ovaries is to be targeted in this program. The larval ovary is connected to the oviduct extended from the external genital anlage during metamorphosis and then forms a functional reproductive system in mature females. Thus, a certain percentage of ovaries transplanted during the larval stage may be incorporated into the reproductive system, followed by mature egg-deposition. Female germ cell differentiation does not yet occur in the ovaries even in mature larvae, and so high tolerance to cryopreservation is expected.
  A first-year goal is technical development to enhance the ovarian transplantation efficiency up to a practical level. It includes the improvement in a manufacturing method for glass needles for transplantation and testing on the usefulness of host larvae without ovaries. A technique for ovary cryopreservation is scheduled to be exploited during the second year.
Project's Progress