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NBRP Genome Information Upgrading Program Project's Progress

Algae

Focus Genome sequencing project of heterocystous cyanobacteria in the NIES collection
Principal Investigator Yuu Hirose  Toyohashi University of Technology
Project Period FY2016
Overview   Cyanobacteria are photosynthetic bacteria that perform oxygen-evolving photosynthesis. In 1990s, the complete genome sequence of Synechocystis sp. PCC 6803 was reported as the first genome of photosynthetic organism (Kaneko et al 1996 Plant Cell Physiol.). Since then, genomes of several cyanobacterial strains have been sequenced, and researchers utilize them as model organisms. However, the spread of the next-generation sequencers in 2010s enables us to study much more diverse cyanobacterial strains deposited in the culture collections (Shih et al 2013 Proc. Natl. Acad. Sci.).
  We focused on the filamentous cyanobacteria that are capable of forming heterocysts. Heterocysts are differentiated cells to fix nitrogen during nitrogen starvation. The genome sizes of heterocystous cyanobacteria are 6-12 Mbp, which are much larger than other non-heterocystous cyanobacteria. In this program, we selected 20 strains of heterocystous cyanobacteria of the National Institute of Environmental Science (NIES) culture collection in Japan (Fig. 1). Their genomes are sequenced and assembled in Toyohashi University of Technology. Annotation and data release are performed in National Institute of Genetics. This program will facilitate both basic and applied studies using cyanobacteria.
Project's Progress