|Focus||Genome sequencing project of heterocystous cyanobacteria in the NIES collection|
|Principal Investigator||Yuu Hirose Toyohashi University of Technology|
Cyanobacteria are photosynthetic bacteria that perform oxygen-evolving photosynthesis. In 1990s, the complete
genome sequence of Synechocystis sp. PCC 6803 was reported as the first genome of photosynthetic organism (Kaneko
et al 1996 Plant Cell Physiol.). Since then, genomes of several cyanobacterial strains have been sequenced, and
researchers utilize them as model organisms. However, the spread of the next-generation sequencers in 2010s enables
us to study much more diverse cyanobacterial strains deposited in the culture collections (Shih et al 2013
Proc. Natl. Acad. Sci.).
We focused on the filamentous cyanobacteria that are capable of forming heterocysts. Heterocysts are differentiated cells to fix nitrogen during nitrogen starvation. The genome sizes of heterocystous cyanobacteria are 6-12 Mbp, which are much larger than other non-heterocystous cyanobacteria. In this program, we selected 20 strains of heterocystous cyanobacteria of the National Institute of Environmental Science (NIES) culture collection in Japan (Fig. 1). Their genomes are sequenced and assembled in Toyohashi University of Technology. Annotation and data release are performed in National Institute of Genetics. This program will facilitate both basic and applied studies using cyanobacteria.